You talk about perceived effects of artificial gravity from rotation when moving with and against that rotation. This is all well known. I also don't see a question. What it your point?

I have not seen it before. Yes, it should be obvious when you start to think about; but I don't recall it being explored in Rama or Babylon 5 (the two uses of O'Neill cylinders that I am familiar with).

There are other effects that will probably be more obvious to the inhabitants than the difference in gravity when moving with or against the rotation. Think about what happens when someone jumps in place, or two people throwing a ball back and forth. However, these things are again obvious and well known.

A flood of "Questions" that come down to "Looky me world, I done figgerd out this effect from high school physics!" would get rather tedious. There has to be a minimum bar for something not to be a dumb question. I'd say high school physics is the absolute minimum, although first semester freshman college physics would be better.

I remember one Babylon 5 episode where Sheridan(?) somehow ended up floating in the interior of the rotating cylinder. They correctly talked about how he's fine, but after eventually drifting to the exterior, he'd effectively slam into things moving 50 miles/hour sideways to him. Koch got out of his encounter suite and flew up to save Sheridan, which finally revealed that Vorlons were what humans thought of as angels.

You are right, though. I don't remember mention of any other effects of the rotating habitat, let alone them being plot points. The "lower levels" were a thing, but it was never mentioned that the apparent gravity there was higher.